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| Registros recuperados: 89 | |
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| Bortoluzzi,Edson C.; Rheinheimer,Danilo dos S.; Gonçalves,Celso S.; Pellegrini,João B.R.; Zanella,Renato; Copetti,André C.C.. |
| Realizou-se este trabalho com o intuito de quantificar a presença de moléculas de agrotóxicos em águas superficiais de três unidades paisagísticas (UP) com diferentes configurações de uso, numa microbacia hidrográfica de cabeceira em Agudo, RS. Dois coletores semi-automáticos de água foram instalados no córrego de cada UP, onde se coletaram amostras, por ocasião de chuvas, em três épocas da cultura do fumo: transplante, aterramento junto aos pés da cultura (planta com 6-10 folhas) e colheita. Os princípios ativos imidacloprid, atrazina, simazina e clomazone foram quantificados por Cromatografia Líquida de Alta Eficiência com Detecção no Ultravioleta (HPLC-UV). Clorpirifós, flumetralin e iprodione foram analisados por Cromatografia Gasosa com Detecção por... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SIG; Erosão; HPLC; Paisagem; Fumo. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662006000400015 |
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| Morales-Trejo,F; Vega y León,S; Escobar-Medina,A; Pérez-González,J.J; Urbán-Carrillo,G; Gutiérrez-Tolentino,R. |
| Se desarrolló una metodología para la determinación de clenbuterol en hígado de bovino por cromatografía líquida de alta resolución (HPLC). La extracción se realizó con acetonitrilo e isopropanol. El extracto se analizó empleando una columna de fase reversa C18, un detector UV a 214 nm y una mezcla de NaH2PO4 0.05M (pH 3.0)/acetonitrilo (85:15, v/v) como fase móvil. El tiempo de retención para el clenbuterol fue 24.82 min. La recta de mejor ajuste de la curva de calibración del estándar fue y = 1340.8563x - 1539.7319, con un r²=0.9997 (p<0.05). Los límites de detección y de cuantificación mostraron valores de 0.9 y 1.8 ng/mL, respectivamente. Las medias de recuperación de clenbuterol fueron de 111.7, 82.0 y 84.8%, para tres niveles de concentración... |
| Tipo: Journal article |
Palavras-chave: Clenbuterol; HPLC; Validación; Hígado de bovino. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100006 |
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| Andrade,E.H.P.; Souza,M.R.; Fonseca,L.M.; Penna,C.F.A.M.; Cerqueira,M.M.O.P.; Roza,T.; Seridan,B.; Resende,M.F.S.; Pinto,F.A.; Villanoeva,C.N.B.C.; Leite,M.O.. |
| Cheese whey level and caseinomacropeptide (CMP) index of fermented milk beverages added with four levels of cheese whey (0, 10, 20, and 40%) and stored at 8-10oC for 0, 7, 14 and 21 days were determined by high performance liquid chromatography-gel filtration (HPLC-GF). Additionally, the interference of the starter culture and the storage time on the detection of cheese whey and CMP were investigated. Refrigerated storage up to 21 days did not affect (P>0.05) cheese whey and CMP amounts in milk (0% of cheese whey) and in fermented milk beverages added with 10 and 20% of cheese whey (P>0.05). However, cheese whey and CMP amounts were higher than expected (P<0.05) in fermented milk beverages added with 40% of cheese whey and stored for 21 days. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fermented milk beverages; Cheese whey; CMP; HPLC. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300959 |
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| Lasmar,M.M.; Leite,M.O.; Fonseca,L.M.; Souza,M.R.; Cerqueira,M.M.O.P.; Penna,C.F.A.M.; Couto,C.N.B.; Ferreira,J.M.. |
| High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Raw milk; Bronopol; Cheese whey detection; HPLC. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600037 |
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| Escobar,A; Abeledo,María Antonia; Vega,E; Cedeño,V. |
| Un procedimiento se desarrolló por cromatografía líquida de alta resolución (HPLC) para determinar Aivlosin en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con acetonitrilo y se concentró cinco veces cuando fue inferior a 20 ppm. El extracto se analizó por HPLC con una columna de fase reversa C18, un detector UV (280 nm) y una mezcla de acetonitrilo: acetato de amonio 0.15M: ácido acético en una proporción de 45:45:10 v/v/v, como fase móvil. Los resultados de la validación mostraron una linealidad entre 5-80 µg/ml, que responde a una ecuación general de Y=-2.60+1.72X con un coeficiente de regresión de 0.999(p<0.05) y una variación del intervalo de confianza de la pendiente entre... |
| Tipo: Journal article |
Palavras-chave: Aivlosin; HPLC; Piensos. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200008 |
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| Escobar,A; Oliva,Yuleivys; Abeledo,María Antonia; Vega,E; Cedeño,V. |
| Se desarrolló un procedimiento por Cromatografía Líquida de Alta Resolución (HPLC) para determinar clortetraciclina en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con una solución de acetona ácida. El extracto se analizó por HPLC con una columna de fase reversa C18 y un detector UV (340 nm) con una mezcla de dimetilformamida al 27% como fase móvil. Los resultados de la validación mostraron una linealidad entre 50-1000 µg/mL que responde a una ecuación general de Y=0.23X-2.62 con un coeficiente de regresión de 0.996 (p<0.05) y una variación del intervalo de confianza de la pendiente entre 0.23-0.24. El límite de detección y el de cuantificación fue de 33µg/g y 50 µg/g... |
| Tipo: Journal article |
Palavras-chave: Clortetraciclina; HPLC; Pienso. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200009 |
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| Lampert, Luis; Hernandez Farinas, Tania. |
| La demande de la part de la Commission Européenne, pour inclure un Indice de Compositions (IC) dans l’indicateur « phytoplancton » de la Directive Cadre de l’Eau, a été prise en compte et un test en conditions réelle a été mené sur les données pigmentaires acquises entre 2016 et 2017. Deux échantillons par mois ont été analysés par chromatographie liquide (HPLC) sur 16 points, situés de la frontière belge et jusqu’au nord de la Loire. Cet indice (ICBC) a été bâti par la comparaison des moyennes pigmentaires de chaque point à une référence, via l’indice de similarité de Bray-Curtis. Cette référence (REF-Atl) a été définie comme la moyenne des deux points les moins impactées (Querqueville et Les Hébihens). La fréquence d’échantillonnage retenue est celle de... |
| Tipo: Text |
Palavras-chave: MANCHE; ATLANTIQUE; DCE; HPLC; PHYTOPLANCTON; INDICE DE COMPOSITION; ICBC. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00469/58110/60530.pdf |
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| Mousavi,Syed Husain Hashemi. |
| A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610 |
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| Mallhi,Tauqeer Hussain; Qadir,Muhammad Imran; Khan,Yusra Habib. |
| In-vitro investigation of Morus nigra L. (Moraceae) has demonstrated the evidence of several antioxidant compounds. Current study was aimed to determine the phytoconstituents and hepatoprotective potential of n-hexane extract of Morus nigra. Swiss albino mice were divided into five groups: normal control, paracetamol control, silymarin control, 250 mg/kg p.o. extract for 7 days plus paracetamol 3 h later, 500 mg/kg p.o. extract for 7 days plus paracetamol 3 h later. Serum levels of liver enzymes and total bilirubin were assessed and compared between the groups by using one-way ANOVA to confirm hepatoprotective activity. HPLC analysis revealed the presence quercetin, oleanolic acid, luteolin, apigenin, vitamin C and kuwanon C. n-hexane extract of Morus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Moraceae; HPLC; Quercetin; Morus nigra/antioxidant; Morus nigra/extract/hepatoprotective. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300631 |
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| Tariq,Mohammad; Thomas,Shindu; Singh,Anu; Talegaonkar,Sushama. |
| Present work is aimed to develop a simple, sensitive, robust and reliable HPLC method for routine quality control of epirubicin (EPI) in bulk drug, marketed injections and polymeric nanoparticles. Separation was carried out by C18 column. Isocratic elution was carried out using mobile phase A: 0.16% o-phosphoric acid solution, B: acetonitrile and methanol mixture (80:20, v/v) in the ratio of 60:40 (A: B) while the flow rate was maintained at 1mL/min. Analyses were performed at 233.5 nm using PDA detector. Excellent linear relationship was observed between peak-area versus drug concentration in the range of 1.0-100.0 μg/mL (r2, 0.999). Developed method was found to be sensitive (Limits of detection and quantification were found to be ~8 ng/mL and ~25 ng/mL,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epirubicin; HPLC; Polymeric nanoparticles; Quality control; Forced degradation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400619 |
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| Sher,Muhammad; Ahmad,Maria; Hassan,Faiza; Naeem-ul-Hassan,Muhammad; Hussain,Muhammad Ajaz. |
| A reverse phase high performance liquid chromatography method has been developed and validated for accelerated stability study and determination of pharmacokinetic parameters of venlafaxine HCl. The chromatographic separation was carried out using ODS analytical column (250 × 4.6 mm i.d., 5 µm particle size). The mobile phase included acetonitrile, methanol and potassium dihydrogen phosphate buffer (30:30:40; pH 6.1) at a flow rate 1.5 mL min−1. UV-Visible detector was used at wavelength of 227 nm to monitor elutions. Retention time observed was 2.745 min. The method was validated for linearity, accuracy, precision, sensitivity and robustness. Accelerated stability study of venlafaxine HCl capsules was carried out at 40 and 50 ºC under 75% RH level.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venlafaxine; HPLC; Stability; Pharmacokinetics; Chromatography. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100514 |
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| Guzmán,Eduardo Lozano; González,José Cruz Calderón; Flores,Maribel Cervantes; Carrillo,Ada Sandoval; Pescador,María Guadalupe Nieto; Cruz,Francisco Javier Moreno. |
| Cnidoscolus chayamansa is a native plant of the Mayan region, which is also cultivated in other places like northern Mexico, Tunisia and India. Many properties are attributed to Mayan Chaya, such as aid in the control of glycemia in diabetics. Thus this study aimed to evaluate the hypoglycemic effects of chaya aqueous extracts in a model of streptozotisin-induced diabetic Wistar rats. Chaya aqueous extracts were collected from plants cultivated in Quinta Roo (Mayan region) and Durango (northern Mexico), and in this study we compare their effect with metformin (as a control). Additionally, we compared the extracts mass profiles from both regions by high-resolution liquid chromatography coupled to a triple quadrupole tandem mass detector (HPLC-MS/MS QQQ).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes; Chaya; Langerhans islets; Hypoglycemia; HPLC. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100619 |
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| Moura,Guilherme de Souza; Lanna,Eduardo Arruda Teixeira; Filer,Keith; Falkoski,Daniel Luciano; Donzele,Juarez Lopes; Oliveira,Maria Goreti de Almeida; Rezende,Sebastião Tavares de. |
| The effects of enzyme complex SSF (solid state fermentation) on growth performance and the availability of sucrose and monosaccharides in the chyme of Nile were involved. The study included 360 fish (70g±4.43) in a completely randomized design with six dietary treatments (0, 50, 100, 150, 200 and 250 ppm of SSF) arranged in six replicates, with 10 fish per replicate. Every 15 days, one tilapia of each experimental unit was sacrificed for analyses of carbohydrate in the chyme. On day 60 of the experiment, the performance parameters were measured. There was a linear effect according to treatment for final weight and weight gain. For the other performance parameters, there were no differences. There was quadratic effect for sucrose and glucose in function of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Enzymes; Fish nutrition; HPLC; Oreochromis niloticus. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000001 |
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| Carrão-Panizzi,Mercedes Concórdia; Beléia,Adelaide Del Pino; Kitamura,Keisuke; Oliveira,Maria Cristina Neves. |
| The effects of genetics and environmental factors on isoflavone content of soybean (Glycine max L.) cultivars grown in different locations in Brazil in 1993/94 were evaluated. Seeds of different cultivars were analised by high performance liquid chromatography (HPLC). In Rio Grande do Sul (RS), Paraná (PR), and Mato Grosso do Sul (MS) States, a significant difference in the isoflavone total content average of the cultivars IAS 5 and FT-Abyara (163.9, 116.4 and 79.5 mg/100 g, respectively) was observed. In general, IAS 5 contained higher isoflavone than FT-Abyara. Cultivars IAS 5 and FT-Abyara grown at Vacaria, RS (28°30' S latitude) with temperature average of 19°C, had the highest isoflavone concentrations (218.7 and 163.8 mg/100 g, respectively). In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Daidzin; Genistin; Crop location; HPLC. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000004 |
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| Cartes Riquelme,Pricila; Ríos Leal,Darcy; Sáez Carrillo,Kátia; Uribe Moraga,Matilde; Valenzuela Aguilar,Sofía; Joseph Bolus,Stephen; Sánchez Olate,Manuel. |
| Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification.... |
| Tipo: Journal article |
Palavras-chave: Somatic embryogenesis; Maturation; Germination; HPLC. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007 |
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| Gutiérrez,R; Vega,S; Pérez,J.J; Ruiz,J.L; Yamazaki,A; Rivera,J.G; Urbán,Georgina; Escobar,A. |
| Con la finalidad de demostrar la inocuidad de la leche orgánica producida en Tecpatán, Chiapas, México, se determinó la presencia de aflatoxina M1 por cromatografía líquido de alta resolución (HPLC). Los resultados mostraron que el 23.3 % de las muestras sobrepasó el límite máximo de residuo propuesto por la regulación mexicana de 0.5 µg kg-1, mientras el 62.7% sobrepasaba el valor de 0.05 µg kg-1 de la UE. Los valores medios de aflatoxina M1 encontrados en la leche durante las épocas de seca y lluvia fueron de 3.53± 0.55 µg kg-¹ y 0.17 ± 0.13 µg kg-1 respectivamente. Los resultados corroboran que los productos orgánicos pueden estar expuestos a la presencia de contaminantes cuando no se cumplen las buenas prácticas agrícolas, afectando su inocuidad. |
| Tipo: Journal article |
Palavras-chave: Aflatoxina M1; Leche orgánica; México; HPLC. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100005 |
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| Registros recuperados: 89 | |
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